Written by the international community's leading experts, Trace Elements in Laboratory Rodents describes the best and most current methods to provide deficient or supplemental trace elements to laboratory animals, as well as how to assay them. The experts warn of the common pitfalls and hidden problems in nutritional testing and how to avoid them. This how-to approach focuses on the technical details that make good, reliable studies. Common as well as rare or recently recognized minerals are described relating to both dietary supplementation and measurement in tissues.
If you are a researcher, professor, or student working in nutrition, food science, biochemistry, or veterinary medicine, you can't afford to be without this excellent hands-on methods manual!
Table of Contents
AIN-93 Purified Diets for the Study of Trace Elements Metabolism in Rodents, P.G. Reeves
Basic Tissue Preparation for Electron Microscopy Assessment of Rodents, R. Wildman
Dietary Iron: Deficiency or Excess, S.M. Smith and H.C. Lukaski
The Use of Iron-Dextran to Produce Iron Overload in Rodents, P. Carthew and A.G. Smith
Extraction and Analysis of Iron Species in Diet and Gut, R.J. Simpson, C. Oguakoya, and T.J. Peters
Manganese Deficiency and Excess in Rodents, J.W. Finley and P.E. Johnson
Manganese Uptake in Tissues In Vitro: Tissue Slices as Models, G.D.V. van Rossum and T. Galeotti
Selenium Diets: Deficiency and Excess, M. Christensen
Selenium in Tissue Culture, C.A. Wardle and A. Shenkin
Copper Deficient and Excess Diets: Theoretical Considerations and Preparations, M.J. Werman and S.J. Bhathena
Copper in Tissue Culture, E. Harris, E. Tiffany-Castiglioni, and Y. Qian
Zinc Diets: Deficiency and Excess, M. Panemangalore and F.N. Bebe
Zinc Radiotracer in the Study of the Mechanisms of Zinc Homeostasis, D. Oberleas
Other Trace Elements
Dietary Boron Deficiency and Supplementation, C.D. Hunt
Measurements of Boron in Rodent Diets and Tissues, C.D. Hunt
Methods for Studying Dietary Lead and its Toxicity in Rodents, J.D. Bogden
Methods in Chromium Dietary Supplementation and Deficiency, G.S. Morris, M. Hegsted, and D.L. Hasten
Isolation and In Vitro Analysis of Biologically Active Chromium, D.M. Stearns
Calcium Bioavailability Using a Rat Model, M.A. Bock and C. Weaver
Vanadium Quantitation, Essentiality, and Pharmacologic and Toxicological Studies in Rodents, B.D. Martin, R.A. Felty, D.M. Stearns, and K.E. Wetterhahn
Nickel-Low Diet Formulation and Tissue Nickel Measurement, E.O. Uthus
"...a 'how-to-do book' that weould be a welcome addition in any nutritional or toxicological scientist's lib rary....Ronald Watson has done a marvelous job in assembling 21 detailed chapters into a comprehensive reference....a great text for those who need a resource that focuses on the details. This book will serve as a key reference resource for the researcher, nutritionalist, toxicologist, and veterinary or animal care person. In addition, it could serve well as a supplemental book for a graduate course on nutrition and trace elements."
-Environmental Nutrition International
"Designed for the researcher with a focus on laboratory and field nutritional research."
- Ira Wolinsky, Series Editor