1st Edition

Molecular and Cellular Methods in Developmental Toxicology





ISBN 9780849333422
Published September 26, 1996 by CRC Press
304 Pages

USD $170.00

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Book Description

This new manual provides a convenient source of experimental procedures, including the most modern and frequently used molecular and cellular techniques. Experimental protocols have been carefully selected by developmental toxicologists for developmental toxicologists. The most important new trends, such as evaluation of the safety of therapeutic antisense oligonucleotides, studies of the role of cell death in abnormal development, and the identification of sparingly expressed developmental control genes are featured. This is the perfect manual for scientists trained in classical developmental toxicology who want to add molecular and cellular methods to their research.

Table of Contents

Preface
Molecular Techniques
Molecular Techniques for Developmental Toxicology: An Introduction, G.P. Daston
Visualization of Gene Expression Patterns by In Situ Hybridization, U. Albrecht, G. Eichele, J.A. Helms, and H.-C. Lu
Utilizing Transgenic Mice to Study Gene Regulation and Function, M.R. Blackburn and R.E. Kellems
The Use of Antisense Oligonucleotide Technology and Mouse Whole-Embryo Culture to Study Gene Function During Organogenesis, L.F. Potts and T.W. Sadler
Nucleic Acid Amplification Technologies, R.H. Finnell, S.J. Vacha, and S.A. Mackler
Protein Identification
Fundamentals of Protein Identification: Biochemical and Immunological Analysis of Developmentally Altered Protein Expression, G.B. Grunwald
Immunohistochemistry, P.E. Mirkes and S. Little
Cellular Techniques
Cellular Techniques for Teratological Cell Death, T.B. Knudsen
Applications of Flow Cytometry in Developmental and Reproductive Toxicology, K.H. Elstein and R.M. Zucker
Modern Techniques for Cell Labeling in Avian and Murine Embryos, D.K. Darnell and G.C. Schoenwolf

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