1st Edition

Lipid Analysis




ISBN 9781872748986
Published July 14, 1996 by Garland Science
192 Pages

USD $74.95

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Book Description

There is increasing recognition of the diversity and biological importance of lipids. Lipid modifications of other biological molecules are now also the subject of intense research activity. This 'user-friendly' introduction describes the techniques curre

Table of Contents

Abbreviations -- Preface -- 1. Introduction -- 2. Basic Techniques -- Extraction methods -- Lipid binding in tissues -- Solvents for lipid extraction -- Some precautions -- Storage of lipid extracts -- Saponification -- Chromatographic procedures -- General considerations -- Partition chromatography -- Adsorption chromatography -- Ion exchange chromatography -- Complexing chromatography -- Spectrometric methods -- UV-visible spectrometry -- IR spectroscopy -- NMR spectroscopy -- Mass spectrometry -- Radioisotopic methods -- Choice of radioisotope -- Autoradiography -- Scintillation counting -- Gas ionization detection -- Gamma counting -- Radioisotopes in lipid studies -- RIA and related techniques -- Immunochemical methods -- Antibodies to lipids -- Hemagglutination in detection and assay -- Immunostaining and TLC -- Immunoradiometric assays -- Enzyme-linked immunosorbent assays -- Analytical methods involving enzymes -- Phospholipases -- Stereospecific analysis of triacylglycerol -- Enzymes in quantifying lipids -- Presentation of lipids in aqueous systems -- Care and safety during the analysis of lipids in biological materials -- General considerations -- Chemical hazards -- Fire hazards -- Radioisotope hazards -- Biological hazards -- Other hazards -- Personal preparedness and protection -- References -- 3. Hydrocarbons -- Biological significance -- Structures -- Detection -- Isolation and purification -- Quantitation -- References -- 4. Alcohols, Phenols, Aldehydes, Ketones and Quinones -- Alcohols and phenols -- General and biological significance -- Structures -- Detection -- Isolation and purification -- Quantitation -- Aldehydes and ketones -- General and biological significance -- Structures -- Detection -- Isolation and purification -- Quantitation -- Quinones -- General and biological significance -- Structures -- Detection -- Isolation and purification -- Quantitation -- References -- 5. Fatty Acids and Prostaglandins -- Fatty acids -- Types of fatty acids found naturally -- Separation methods for fatty acids -- GLC of fatty acids -- HPLC of fatty acids -- Prostaglandins and leukotrienes (eicosanoids) -- Analysis of eicosanoids -- References -- 6. Esters -- Acylglycerols -- Separation of acylglycerols -- Analysis of acylglycerols -- Fatty acids of acylglycerols -- Wax esters -- Alkylglycerols -- Analytical methods for alkylglycerols -- References -- 7. Phospholipids, Sulfolipids and Related Compounds -- Phospholipids with amino-groups -- Hydrolytic procedures in analysis -- Estimation of phosphate -- Separation of phospholipids -- Phosphoinositides -- Phosphoinositide and inositol phosphate metabolism -- Separation of the phosphoinositides -- Receptor-linked phosphoinositide changes -- Separation and estimation of inositol phosphates -- Estimation of diacylglycerol -- Phosphatidylinositol glycans -- Detection of PI glycans -- Phosphonolipids -- Separation and analysis of phosphonolipids -- Alkylether phospholipids -- Analysis of alkylether phospholipids -- Sulfolipids -- Lipid sulfates -- Sulfonolipids -- Analytical methods -- References -- 8. Glycolipids -- Glycosphingolipids -- Biological significance -- Structures -- Detection -- Isolation and purification -- Quantitation -- Polyisoprenyl phosphosugars -- Biological significance -- Structures -- Detection -- Isolation and purification -- Quantitation -- Glycosylglycerides and related compounds -- Biological significance -- Structures -- Detection -- Isolation and purification -- Quantitation -- Phosphoglycolipids, lipoteichoic acids and related compounds -- Biological significance -- Structures -- Detection, isolation, preparation and quantitation -- References -- 9. Lipoproteins -- Plasma lipoproteins -- Structures and biological significance -- Detection -- Isolation and purification -- Quantitation -- Fatty acyl proteins and prenyl proteins -- Biological significance -- Structures -- Detection -- Isolation and purification -- Quantitation -- References -- Appendices -- Appendix A: Glossary -- Appendix B: Suppliers -- Appendix C: Further reading -- Index.

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Author(s)

Biography

Department of Biochemistry, Medical School, Queen's Medical Centre, Nottingham NG7 2UH, UK. Department of Biochemistry, Medical School, Queen's Medical Centre, Nottingham NG7 2UH, UK.

Reviews

"Lipid Analysis is an inexpensive and well-produced book, with many clear figures and line drawings to illustrate separations." Trends in Food Science and Technology November 1996 "It is a thorough-going account at a research level (which is) extremely we