Completely revised to reflect the innovations in HPLC from the past decade, this authoritative reference presents practical strategies for the evaluation and analysis of proteins, peptides, and polynucleotides. Offering class-specific applications for the characterization and fractionation of biological macromolecules, the book contains material on organic supports, size exclusion, ion exchange, hydrophobic interaction, and metal interaction chromatography. Leading experts summarize specialized detection systems, provides discussions on the chemical and biological properties of specific biomolecules, include detailed guidelines for the development of analytical techniques, and more.
Table of Contents
THE TECHNIQUE. Silica as a Support in HPLC of Biomacromolecules. Organic Polymer Support Materials. Size Exclusion Chromatography. Ion-Exchange Chromatography. Reversed Phase and Hydrophobic Interaction Chromatography of Proteins and Peptides. Metal Interaction Chromatography. Preparative Chromatography. Sample Preparation. Gradient Elution Separation of Large Biomolecules. CLASS SPECIFIC APPLICATIONS. Analytical HPLC of Peptides. HPLC of Membrane Proteins. HPLC of Cereal Endosperm Storage Proteins. Analysis of Hemoglobin Variants and HbA1c, U. Turpeinen. Antibodies. Glycoproteins. DETECTION METHODS. Immunodetection of Proteins in High-Resolution Separation Systems. Characterization of Proteins, Peptides, and Polynucleotides by Mass Spectrometry. Detection and Analysis of Proteins by High Performance Liquid Chromatography with Photodiode Array Detection. Index.
Gooding, Karen M.; Regnier, Fred E.